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. 2018 Jul 20;8:10978. doi: 10.1038/s41598-018-29379-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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An example of correlated finding of an isolated, focal micro calcification in the internal elastic lamina (IEL). (A) Tomographic cross-sectional image of the intact coronary segment shows a bright dot in mid-wall as outlined in square. Scalebar is 1 mm. (B) Histological image of the section at the same level in low magnification shows a faint brown stain at the same location outlined in square. Scalebar is 1 mm. (C) Magnified view of the outlined area in panel A shows a bright (high density) dot. Scalebar is 100 µm. (D) Magnified view of the corresponding area in the histological image shows a focal calcification at the same location, which appears dark brown by the von Kossa stain. Scalebar is 100 µm. (E) Further magnification of the histological image shows the location of the micro-calcification in the IEL. Scalebar is 25 µm.