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. 2018 Jul 20;8:11011. doi: 10.1038/s41598-018-29116-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Standard curves of the target genes. The standard curve can be used as an accurate quantification of unknown samples. The standard curve R² of the target gene was greater than 0.99, the slope K value satisfies |ΔK| ≤ 0.1 (Table S6). qRT-PCR was therefore performed using the ΔΔCT method. Biology duplications are three times.