Table 1.
Reaction system of qPCR.
| Reagent | Volume |
|---|---|
| SYBR Premix Ex TaqTM (2×) | 10 μL |
| Forward primer (10 μmo1/L) | 0.4 μL |
| Reverse primer (10 μmo1/L) | 0.4 μL |
| Rox | 0.4 μL |
| ddH2O | 6.8 μL |
| cDNA | 2 μL |
| Total | 20 μL |
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