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. 2018 Jul 20;8:10973. doi: 10.1038/s41598-018-29384-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Coelomic fluid of sample B. Evaluation of CF for sex determination by quantitative fluorescent PCR. Electropherograms of amplification product from CF without maternal contamination using small tandem repeat (STR) markers specific for chromosome Y. A part of the total electropherograms are displayed in Panel A–C (A) coelomic cell pattern; (B) foetal tissue pattern; (C) maternal pattern. AMXY: are visible two different peaks, one peak of 105 bp specific for X chromosome, and one of 110 bp specific for Y chromosome. DXS1187: STR located in the X chromosome. Coelomic cell DNA and foetal DNA showed only one peak of maternal origin. HPRT: STR located in the X chromosome. Coelomic cell DNA and foetal DNA showed only one peak of maternal origin. SRY: STR located in the Y chromosome, absent in the profile of the mother and present as one peak in the profiles of coelomic cell DNA and foetal DNA.