Fig. 1.

AIMD depletes the gut microbiome. a Stool cultures from AIMD mice yielded fewer colonies, n = 5/group. For box plot, center is mean, box is 25th to 75th percentile, and whiskers are 5th to 95th percentile. Kruskal–Wallis test, *p < 0.05, **p < 0.01. b, c 16S results show that AIMD mice had a decrease in OTUs detected from the Firmicutes and Bacteroidetes phyla, and an increase in Proteobacteria phyla, n = 6–8/group, mean percent abundance (with SEM). These differences were significant as assessed by ANCOM²⁴. d 16S results showing the shifts in microbiome in each treatment condition. e Principal coordinate analysis of the gut microbiome, n = 6–8/group. Fecal specimens were collected approximately 2 weeks after intervention