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. 2018 Jul 20;8:10966. doi: 10.1038/s41598-018-29298-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of peptide concentrations and BY-2 cell densities on cell penetration efficiency. (a) CLSM overlay images of BY-2 cells treated with TAMRA-labeled BP100. Red signals indicate TAMRA fluorescence. The peptide concentration ranged from 20 to 150 µg/mL. The cell density ranged from 230 to > 1000 cells/µL. (b) Cell penetration efficiency of BP100 at different peptide concentrations and BY-2 cell densities. The efficiencies were determined using CLSM images. The error bars show standard deviations of three independent experiments (Tukey’s HSD test, *P < 0.05).