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. 2018 Jul 20;8:10966. doi: 10.1038/s41598-018-29298-6

Figure 7.

Figure 7

Infiltration of TAMRA-labeled BP100 (Peptide No. 1) from adaxial to abaxial side of Nicotiana benthamina leaf surface. The BP100 is introduced from abaxial surface of 2-weeks-old N. benthamina leaf by infiltration. After the infiltrated plant were cultured for 3 h at 23 °C in dark, the leaf section was used for CLSM analysis after deairation. (ad) The non-infiltrated and (eh) filtrated leaves (eh) were imaged by CLSM to obtain single images of the TAMRA-labeled BP100 (red) and chloroplast (green). Images show the leaf epidermal (a,c,e,g) and mesophyll cells (b,d,f,h) in both adaxial (a,b,e,f) and abaxial side (c,d,g,h) of the leaves.