Figure 1.

AAV Diffusion in Spontaneously Expectorated CF Sputum

(A) Representative trajectories of AAV1 and AAV6 in sputum samples from 3 CF patients. Trajectories show 10 s of AAV motions. Color bar indicates the time (t) of the trajectory in seconds. The scale bar represents 1 μm. (B) Box-and-whisker plots of MSD_{1 s} of AAV1 and AAV6 in sputum samples collected from 8 individual CF patients are shown. Maximum whisker length is 1.5 times the interquartile range; outliers are shown as dots. (C) Representative trajectories of AAV1, AAV6, and AAV6-K531E in CF sputum are shown. Trajectories show 10 s of AAV motions. Color bar indicates the time (t) of the trajectory in seconds. The scale bar represents 1 μm. (D) Box-and-whisker plots of MSD_{1 s} for AAV1, AAV6, and AAV6-K531E in all patient samples tested are shown. Outliers are not shown. *p < 0.05; Mann-Whitney test.