Figure 2.

In Vitro Transgene Expression in Human CFBE41o^- ALI Cultures

(A–C) Representative images of CFBE41o^- ALI cultures transduced with (A) AAV1, (B) AAV6, and (C) AAV6-K531E. The scale bar represents 50 μm. (D and E) Image-based quantification of AAV-mediated GFP transgene expression in terms of (D) overall level and (E) relative coverage in CFBE41o^- ALI cultures (n = 6) is shown. (F) Mucin contents of CFBE41o^- ALI cultures before and after CSS treatment are shown. (G and H) Representative images of CFBE41o^- ALI cultures transduced with (G) AAV1 and (H) AAV6 where cultures were pretreated overnight with CSS are shown. (I and J) Image-based quantification of AAV-mediated GFP transgene expression in terms of (I) overall level and (J) relative coverage in CSS-pretreated CFBE41o^- ALI cultures (n = 6) is shown. *p < 0.05; Student’s t test (F) or one-way ANOVA (D, E, I, and J).