Figure 4.

In Vivo Transgene Expression in the Lungs of Scnn1b-Tg Mice following Intratracheal Administration of AAV1 or AAV6

(A–H) Representative images of YFP expression (green) in the proximal (A–D) and distal airways/airspace (E–H). Confocal images of the proximal lung tissues from AAV1-treated mice showing cell nuclei only (A) or with YFP transgene expression (B) and from AAV6-treated mice showing cell nuclei only (C) or with YFP transgene expression (D). Confocal images of the distal lung tissues from AAV1-treated mice showing cell nuclei only (F) or with YFP transgene expression (G) and from AAV6-treated mice showing cell nuclei only (G) or with YFP transgene expression (H).Cell nuclei are stained with DAPI (blue). Regions of the airway lumen where mucus plugs were observed are outlined in red. The scale bar represents 200 μm. (I) Image-based quantification of YFP expression coverage is shown (n = 3 mice with 3 or 4 lung sections per mouse). (J) Luciferase expression mediated by AAV1 or AAV6 quantified for whole-lung homogenates is shown (n = 6 mice). *p < 0.05; Student’s t test.