Figure 5.

AP Staining of AAV6- and AAV6.2FF-Transduced Nose and Lungs

1 × 10¹¹ vg of AAV6- or AAV6.2FF-AP was delivered intranasally to C57BL/6 mice (n = 4/group), and tissues were harvested and stained after 3 wk. Representative images of (A and E) the nose and (B–D, F–H) lung lobes for both vectors are shown. Lung sections were counterstained with nuclear fast red, and transduced cells expressing AP appear purple. Representative sections of transduced lung are shown at (I and L) ×20 and (J, K, M, and N) ×40 original magnification for AAV6 and AAV6.2FF.