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Quantification of AAV Vector Genomes in Transduced Lung Tissue
(A) TaqMan qPCR quantification of AAV genome copy number per nanogram of total DNA extracted from paraffin-embedded lung tissue. Values are expressed as means ± SD. Differences in AAV vg copy number between AAV6 and AAV6.2FF were determined using a Student’s t test. (B) Quantification of AP enzymatic activity in unfixed lung tissue. Dashed line indicates the threshold of detection for the assay.
