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. 2018 Jul 20;18:99. doi: 10.1186/s12883-018-1103-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — RT-PCR shows gene profiles on genomic DNA. To determine relative gene load, a ∆∆Cq method was performed from qPCR data with GAPDH as an endogenous reference gene. These data show a depletion of the ZNF511, CALY, PRAP1, FUOM, and ECHS1 genes in two cases (red and purple histograms) with respect to the father (green histogram), who does not present a deletion of the region of interest