Fig. 5.
Effects of Nogo-P4 on phosphorylation of MLC and cofilin and expression of IQGAP in microglia. MLC, cofilin, and IQGAP are key cytoskeleton proteins. After microglia from 3-month-old mice were treated with BSA (0.01% in PBS), fAβ1–42 (10 μM), Nogo-P4 (100 μg/ml), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM) for 6 h, phosphorylation of MLC and cofilin, and expression of IQGAP were quantified using Western blot. MLC (a, b), cofilin (c, d). Values were reported as the mean ± SD. IQGAP (e, f). Values were reported as the mean ± SD, as a percentage of values determined in the BSA group (control, 100%). *p < 0.05, **p < 0.01, ***p < 0.001, when compared with the BSA group; ###p < 0.001, when compared with the fAβ1–42 group, n = 3