Table 5. IPA software prediction of upstream regulators in HCT116 and HepG2 cells that were treated with MP-HX.

IPA predicts modulation of upstream regulators and ranked them based on the activation z-score. A positive score denotes activation, while a negative score denotes inhibition.

Cell line	Activation/Inhibition	UR	MA_FC	z-score	Overlap p-value
HCT116	Activated	NUPR1	+1.27	+10.94	8.34E−57
		TP53	+1.38	+7.16	3.07E−32
		TNF	+1.07	+4.81	1.15E−12
		KDM5B	+2.44	+4.79	9.05E−18
		IL1A	+2.74	+3.86	1.39E−08
		NFkB (complex)		+3.65	2.24E−12
		AR	+1.14	+3.61	4.8E−15
		CDKN1A	+2.36	+3.59	3.36E−10
		TGFB1	−1.20	+3.54	2.48E−21
		E2F6	+1.27	+3.46	1.01E−10
	Inhibited	RABL6	−1.28	−6.25	9.25E−37
		MITF	+1.12	−5.62	3.56E−26
		RARA	−1.24	−5.10	4.43E−11
		ESR1	−1.16	−4.91	9.74E−17
		FOXM1	−2.96	−4.89	2.09E−21
		TAL1	−1.21	−4.15	6.83E−08
		E2F3	−1.35	−3.71	1.99E−14
		MYC	−1.40	−3.67	3.23E−14
		ERBB2	−1.12	−3.54	7.77E−57
		E2f		−3.46	4.87E−19
HepG2	Activated	NUPR1	+2.40	+12.21	1.95E−73
		TP53	+1.34	+6.22	2.45E−27
		TGFB1	+2.29	+5.37	3.98E−17
		PDGF BB		+4.81	2.03E−14
		PRKCD	+1.63	+4.74	9.17E−11
		ERK		+4.68	3.94E−10
		SMARCA4	+1.06	+4.26	2.52E−10
		TREM1	+8.43	+3.75	1.56E−08
		TGM2	−1.34	+3.72	0.0044
		KDM5B	+1.08	+3.54	1.54E−13
	Inhibited	RABL6	−1.11	−4.80	1.15E−24
		RARA	+1.09	−4.40	1.22E−10
		MITF	+1.24	−4.19	7.93E−11
		ESR1	+1.08	−4.04	5.29E−17
		IgG		−3.84	6.49E−07
		26s Proteasome		−3.64	5.77E−14
		Hdac		−3.57	2.64E−08
		KIAA1524	−2.21	−3.26	7.02E−04
		FOXM1	−1.87	−3.20	6.48E−13
		TAL1	−1.22	−3.15	1.66E−06