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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Int J Parasitol. 2018 Jun 6;48(9-10):773–783. doi: 10.1016/j.ijpara.2018.04.002

Fig. 3.

Fig. 3.

Proliferative response of antigen-responding peripheral blood mononuclear cells (PBMCs)) in the blood of vaccinated macaques. (A) PBMCs (1×106) were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) and stimulated with 1 ug/ml of rBmHAT or rBmHAXT (recombinant Brugia malayi HSP12.6+ALT-2+TPX+TSPLEL) or ConA for 5 days. Dividing cells were counted in a flow cytometer. Each data point represents a proliferation index. ‘+’indicates the mean proliferation index for each group. (B) Representative flow cytometer data from the experimental group. Up to eight generations of dividing cells were present in the PBMC cultures that were stimulated with rBmHAXT. n=10 macaques per group. AL007, alum; AL019, alum plus glucopyranosyl lipid adjuvant-stable emulsion; FL1-H, fluorescence relative intensity.