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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Int J Parasitol. 2018 Jun 6;48(9-10):773–783. doi: 10.1016/j.ijpara.2018.04.002

Fig. 4.

Fig. 4.

T cell population and their cytokine profile in the peripheral blood mononuclear cells (PBMCs) from rBmHAT and rBmHAXT (recombinant Brugia malayi HSP/ALT-2/TPX/TSPLEL) vaccinated macaques. PBMCs (1×107) were incubated with rBmHAT or rBmHAXTfor 3 days. Following incubation, cells were stained with fluorescent antibodies and processed for flow cytometry. Cells were initially gated for CD3 and the population of CD4, CD8 and CD28 positive cells was identified as indicated in (A). The CD28 positive cells were then further gated to determine the population of CCR7 positive cells with intracellular IFN-γ or IL-4. (B) Percentage of CCR7+ and CCR7− cells among CD28+ cell populations. (C) Percentage of IFN-γ+ and (D) IL-4+ cells among CCR7-CD28+ cell populations. The values represent percentage of cell population in each group (n = 10 macaques per group). (SC-H, side scatter-height; FSC-H, forward scatter-height; PE, phycoerythrin; TCM, T central memory cells; TEM, T effector memory cells; Interferon IFN)-γ; Interleukin (IL)-4; CD, cluster of differentiation; CCR, C-C chemokine receptor type.