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. Author manuscript; available in PMC: 2018 Jul 22.
Published in final edited form as: Cell Rep. 2018 Jun 12;23(11):3286–3299. doi: 10.1016/j.celrep.2018.05.032

Figure 4. The Autophagy-Hyperactive Becn1F121A Mutation Reduces GSIS.

Figure 4

(A) ELISA analyses of plasma insulin levels 0–30 min after glucose injection in HFD-fed WT and Becn1F121A mice. n = 6–7; one-way ANOVA with Tukey-Kramer test.

(B) Insulinogenic index of WT and Becn1F121A mice fed with HFD for 8 weeks. n = 6.

(C) GSIS in MIN6 β cells stably expressing vector, or hemagglutinin (HA)-tagged WT Becn1 or Becn1F121A mediated by lentivirus, normalized to cell number. Western blot analysis of Becn1 expression in MIN6 cells was shown on the right. n = 5; t test.

(D) Hyperglycemic clamp study of WT and Becn1F121A mice fed with HFD for 9 weeks. Plasma insulin, glucose infusion rate, plasma glucose, and body weight are shown. n = 9–10; t test.

Data represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

See also Figure S7.