Figure 3.

ackA Mutation Is Sufficient to Improve L. plantarum^NIZO2877 Growth-Promoting Effect

(A) Longitudinal size of larvae measured 7 days AED on poor-nutrient diet. Larvae were kept germ-free (GF) or associated with Lp^NIZO2877, Lp^WJL, FlyG2.1.8, or with FlyG2.1.8-reverted strain (FlyG2.1.8^Rev). The Delta in larval size (ΔLS), the difference between the size of larvae associated with the respective L. plantarum strain and the size of larvae associated with Lp^NIZO2877, is shown. FlyG8.2.1 strain, exhibiting a significant difference (improved) at promoting larval growth compared with Lp^NIZO2877 (Student's t test: p < 0.05), is shown in red. Strains that did not exhibit a significant difference (improved) at promoting larval growth compared with Lp^NIZO2877 are shown in white.

(B) Developmental timing (DT) of individuals that were kept GF or associated with Lp^NIZO2877, Lp^WJL, FlyG2.1.8 or with FlyG2.1.8^Rev strain. The minus Delta in developmental timing (−ΔDT) between the mean time of emergence of 50% of the pupae associated with the respective condition and the mean time of emergence of 50% of the pupae associated with Lp^NIZO2877 is shown in the graph. FlyG8.2.1 strain, exhibiting a significant difference (improved) at promoting larval growth compared with Lp^NIZO2877 (Student's t test: p < 0.05), is shown in red. Strains that did not exhibit a significant difference (improved) at promoting larval growth compared with Lp^NIZO2877 are shown in white.