Figure 6.

N-Acetyl-Glutamine Recapitulates the Beneficial Effect of FlyG2.1.8 on Lp^NIZO2877-Associated Larvae

(A) Heatmap showing the metabolites that differ significantly between experimental groups (Lp^NIZO2877 and FlyG2.1.8) (two-sided t tests, p < 0.05). The heatmap was generated with heatmap.2 function in R. The compounds are ordered by the metabolite class given by the left scale.

(B) Longitudinal size of larvae (n > 60 larvae/group) measured 7 days AED on poor-nutrient diet supplemented with different concentrations (g/L) of N-acetyl-glutamine (x axis). Larvae were kept germ-free (GF) or associated with Lp^NIZO2877 (ancestor) and with Fly.G2.1.8 (evolved strain). Larval size is shown as mean ± SEM. ^∗∗∗p < 0.01.

(C) 1:1 competitive assay between Lp^NIZO2877 and Lp^NIZO2877-evolved strain (FlyG2.1.8) in poor-nutrient diet supplemented with 0.1 g/L of N-acetyl-glutamine. Error bars represent the percentage of each strain detected by qPCR. ^∗p < 0.05, ^∗∗∗p < 0.01, obtained by Student's t test.