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Greensporone C dephosphoryalted AKT and suppresses the expression of downstream targets. K562 (A) and U937 (B) cells were treated with increasing doses of GC for 24 h as indicated. After cell lysis, equal amounts of proteins were separated by SDS–PAGE, transfered to PVDF membrane, and immunoblotted with antibodies of p-AKT, AKT, XIAP, c-IAP1, c-IAP2, and GAPDH as indicated.
