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. 2018 Jul 16;9:1609. doi: 10.3389/fimmu.2018.01609

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Copyright © 2018 Zhu, Nemoto, Mailloux, Perez-Villarroel, Nakagawa, Falahat, Berglund and Mulé.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishing a lymph node (LN)-derived stromal cell line. (A) A photomicrograph of a LN-derived monoclonal stromal cell line (#2) in culture. Monoclonal cell lines were generated by limiting dilution. Scale bar denotes 0.2 mm. (B) Total RNA was extracted from the stromal cell line (#2) at 3 different passages and mRNA level of indicated 11 chemokines were analyzed by mouse genome arrays. Log2 transformed data were presented and red bars denote the mean. (C) The stromal cell line was stained for CD3, CD45, CD31, podoplanin, LTβ receptor (LTβR), and vascular cell adhesion molecule 1 (VCAM-1), and analyzed by flow cytometry. The majority of the cells are fibroblastic reticular cells with expression of VCAM-1 and LTβR.