Table 3.

Analysis of variance (two-way ANOVA) for the measured biological parameters as affected by soil origin (O), sampling site (S) and their interactions (O × S).

Parameter	Source of variation	df	Sum of squares	Mean square	Variance explained (%)	F	P
MICROBIAL COUNTS
Oligotrophic bacteria (10 % TSBA + nystatin)	Origin (O)	4	9.95	2.49	69.05	479.98	<0.001***
	Sampling site (S)	2	0.87	0.43	6.02	83.73	<0.001***
	O × S	8	3.44	0.43	23.85	82.91	<0.001***
Zinc-tolerant bacteria (10 % TSBA + 1.5 mM Zn2+ + nystatin)	Origin (O)	4	8.41	2.10	41.84	41.39	<0.001***
	Sampling site (S)	2	6.05	3.02	30.10	59.55	<0.001***
	O × S	8	4.11	0.51	20.48	10.13	<0.001***
Cadmium-tolerant bacteria (10 % TSBA + 0.5 mM Cd2+ + nystatin)	Origin (O)	4	13.21	3.30	25.88	37.21	<0.001***
	Sampling site (S)	2	24.47	12.24	47.96	137.91	<0.001***
	O × S	8	10.68	1.34	20.94	15.05	<0.001***
Fungi (PDA + Rose Bengal + streptomycin)	Origin (O)	4	2.37	0.59	48.26	41.89	<0.001***
	Sampling site (S)	2	0.54	0.27	10.95	19.01	<0.001***
	O × S	8	1.58	0.20	32.16	13.96	<0.001***
PLFA
Total PLFA biomass	Origin (O)	4	23,178.72	5,794.68	36.05	17.17	<0.001***
	Sampling site (S)	2	17,749.87	8,874.93	27.61	26.29	<0.001***
	O × S	8	13,233.07	1,654.13	20.58	4.90	<0.001***
Bacterial PLFA biomass (BB)	Origin (O)	4	3,709.42	927.36	41.83	18.09	<0.001***
	Sampling site (S)	2	1,520.76	760.38	17.15	14.83	<0.001***
	O × S	8	2,099.19	262.40	23.67	5.12	<0.001***
Gram-positive bacteria PLFA biomass (GP)	Origin (O)	4	500.95	125.24	26.51	7.69	<0.001***
	Sampling site (S)	2	550.03	275.02	29.11	16.89	<0.001***
	O × S	8	350.20	43.77	18.53	2.69	0.023*
Gram-negative bacteria PLFA biomass (GN)	Origin (O)	4	1,362.35	340.59	51.74	34.49	<0.001***
	Sampling site (S)	2	200.87	100.43	7.63	10.17	<0.001***
	O × S	8	773.77	96.72	29.38	9.79	<0.001***
GP:GN ratio	Origin (O)	4	0.06	0.02	3.52	1.12	0.364
	Sampling site (S)	2	0.94	0.47	54.43	34.75	<0.001***
	O × S	8	0.32	0.04	18.55	2.96	0.014*
Actinomycetes PLFA biomass	Origin (O)	4	26.73	6.68	26.82	19.26	<0.001***
	Sampling site (S)	2	50.31	25.16	50.50	72.52	<0.001***
	O × S	8	12.19	1.52	12.23	4.39	0.001**
Fungal PLFA biomass (FB)	Origin (O)	4	131.08	32.77	59.62	95.20	<0.001***
	Sampling site (S)	2	47.09	23.54	21.42	68.40	<0.001***
	O × S	8	31.37	3.92	14.27	11.39	<0.001***
BB:FB ratio	Origin (O)	4	1,129.17	282.29	44.86	107.88	<0.001***
	Sampling site (S)	2	542.08	271.04	21.54	103.58	<0.001***
	O × S	8	767.19	95.90	30.48	36.65	<0.001***
BIOLOG-CLPP
∑AUC	Origin (O)	4	1,379,281.030	344,820.258	34.83	135.607	<0.001***
	Sampling site (S)	2	1,394,786.201	697,393.100	35.22	274.263	<0.001***
	O × S	8	1,109,553.371	138,694.171	28.02	54.544	<0.001***
H'	Origin (O)	4	0.581	0.145	21.51	5.810	0.001**
	Sampling site (S)	2	0.379	0.190	14.05	7.588	0.002**
	O × S	8	0.989	0.124	36.67	4.951	0.001**
Rs	Origin (O)	4	84.089	21.022	15.94	3.403	0.021*
	Sampling site (S)	2	59.511	29.756	11.28	4.817	0.015*
	O × S	8	198.711	24.839	37.66	4.021	0.002**
Eh	Origin (O)	4	0.022	0.005	21.72	4.76	0.004**
	Sampling site (S)	2	0.014	0.007	13.99	6.13	0.006**
	O × S	8	0.030	0.004	30.06	3.29	0.008**
DGGE
H'	Origin (O)	4	0.8251	0.2063	76.10	302.0	<0.001***
	Sampling site (S)	2	0.1158	0.0579	10.68	85.0	<0.001***
	O × S	8	0.1228	0.0154	11.33	22.0	<0.001***
Rs	Origin (O)	4	2,940.8	735.2	60.83	624.2	<0.001***
	Sampling site (S)	2	872.1	436.1	18.04	370.2	<0.001***
	O × S	8	986.5	123.3	20.40	104.7	<0.001***
Eh	Origin (O)	4	0.0047	0.0012	43.63	63.0	<0.001***
	Sampling site (S)	2	0.0015	0.0007	13.76	40.0	<0.001***
	O × S	8	0.0040	0.0005	37.45	27.0	<0.001***

H', Shannon-Wiener biodiversity index; Rs, richness; Eh, evenness. Asterisks represent significance level according to ANOVA (

^*P < 0.05,

^**P < 0.01 and

^***P < 0.001).