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. 2018 Jul 16;9:1589. doi: 10.3389/fimmu.2018.01589

Figure 2.

Figure 2

Effect of Apyrase treatment on nephrotoxic serum (NTS)-driven glomerulonephritis (GN). C57BL/6 mice received either vehicle (PBS) or (NTS solved in PBS) intravenous on days 1, 2, and 3 to induce GN. For treatment, they received i.p. injections of either vehicle or apyrase (4 U/ml in 100 µl) on days 1, 3, 5, 7, and 9. Urine was collected daily and analyzed for its adenosine-5’-triphosphate content (A) and its albumin–creatinine ratio (B). Animals were killed on day 10 after a blood sample had been taken. This was analyzed for its serum urea and creatinine levels (C). From the killed animals, kidney tissue was prepared and analyzed with microscopy (PAS-staining). Approximately 100 Glomerula in each sample were analyzed following the histological criteria “normal, crescentic, or thrombotic” (D). Follow-up of mice receiving apyrase or vehicle are shown (E). Data are shown as mean ± SEM, n = 4–5 mice in each group. *P < 0.05. Data from one representative experiment out of three are shown.