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. 2018 Jul 16;9:1589. doi: 10.3389/fimmu.2018.01589

Figure 5.

Figure 5

Effect of P2Y2-deficiency in mice with nephrotoxic serum (NTS)-driven glomerulonephritis (GN). P2Y2-receptor−/− mice or wild-type animals (all C57BL/6 mice) received NTS (solved in PBS) intravenously on days 1, 2, and 3 to induce GN. Urine was collected on days 4, 5, and 9 and analyzed for its albumin–creatinine quotient (A). Animals were killed on day 10 after a blood sample had been taken. This was analyzed for its serum urea and creatinine levels (B). From the killed animals, kidney tissue was prepared and analyzed with microscopy (PAS-staining). Approximately 100 Glomerula in each sample were analyzed following the histological criteria “normal, crescentic, or thrombotic” (C). We also performed a survival experiment following the same experimental setup (D). Data are shown as mean ± SEM, n = 5 mice in each group. *P < 0.05. Data from one representative experiment out of two are shown.