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. 2018 Jul 16;9:773. doi: 10.3389/fphar.2018.00773

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Copyright © 2018 Youn, Park, Kim, Jung, Kang, Lim, Song, Kwak and Um.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of BBR on BPH-related factors in TP-treated RWPE-1 cells. (A) Cell viability was assessed by an MTS assay. Values are mean ± SD of 10 or more separate experiments ^∗P < 0.05 when compared to TP. (B) Representative photomicrographs of EdU assay of TP-treated RWPE-1 cells are shown. (C) The relative protein expressions of PSA, AR, and 5AR were analyzed by a Western blot analysis. Values are mean ± SD of three or more separate measurements. ^#P < 0.05 when compared to NC; ^∗P < 0.05 when compared to BPH. The protein expressions differences were normalized to GAPDH. IF staining of (D) PSA (red), (E) AR (red), and (F) 5AR (green) merged with DAPI (blue) was performed. NC, normal control group; TP, TP-treated group; BBR, BBR-treated group.