Table 3.
Material list for retrograde motor neuron labeling with carbocyanine dyes for rats.
| Name | Type | Company | Catalog Number | Comments |
|---|---|---|---|---|
| Isoflurane | Reagent | Abbot | Anesthesia | |
| DiO | Reagent | Thermo Fisher Scientific | D275 | Retrograde tracer (Green) |
| DiI | Reagent | Thermo Fisher Scientific | D282 | Retrograde tracer (Red) |
| DiD | Reagent | Thermo Fisher Scientific | D307 | Retrograde tracer (Far red) |
| DMF | Reagent | Wako | 050-05821 | Solvent |
| EtOH | Reagent | Wako | 057-00451 | Solvent |
| PermaFluor | Reagent | Thermo Fisher Scientific | TA-030-FM | Aqueous mounting medium |
| 25 μl Hamilton syringe | Supply | Hamilton | 80401 | Luer Tip |
| Vibratome | Equipment | Leica Microsystems | VT1000S | Vibrating blade microtome |