. 2018 Mar 30;5:283–298. doi: 10.1016/j.mex.2018.03.007

Table 4.

Material list for retrograde motor neuron labeling with cholera toxin B subunit for mice.

Name	Type	Company	Catalog Number	Comments
Ketamine	Reagent	Daiichi-Sankyo	KETALAR	Anesthesia: Strictly regulated.
Xylazine	Reagent	Sigma-Aldrich	X1251	Sedating drug
Cholera Toxin B subunit	Reagent	Sigma-Aldrich	C9905	Retrograde tracer. Need documentation to purchase.
Microneedle syringe	Supply	WPI	NANOFIL	Comes with 26 gauge needle.
Cryo-blocking medium	Supply	Sakura Finetek	Tissue-Tek^TM O.C.T. compound^TM	Transparent
Cryomold	Supply	Sakura Finetek	Tissue-Tek^TM Cryomold^TM	10 mm × 10 mm × 5 mm
Blocking serum	Reagent	Vector Lab	S-5000	Normal rabbit serum
1^st antibody	Reagent	List Biological Lab	703	Go anti-CTB
				RRID:AB_10013220
2^nd antibody	Reagent	Vector Lab	BA-5000	Rb anti-Go IgG, biotinylated
Streptavidin	Reagent	Thermo Fisher Scientific	S21375	Alexa Fluor 633-conjugated
Fluoro Nissl solution	Reagent	Thermo Fisher Scientific	N-21470	Blue
Cryostat	Equipment	Leica Microsystems	CM1860	Freezing microtome