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. 2018 Jul 16;9:1557. doi: 10.3389/fmicb.2018.01557

Table 2.

Summary of the Sanger sequencing results comparing the product (GYO reishi kits and manufactured reishi products) taxonomy labels to the DNA-based barcode identification.

Sample #1 Product2 Product Label3 Taxon Identified4 GB ITS/tef1α Accession
AL-M1sd GYO reishi kit G. lucidum G. sessilech MH160056/MH168053
AL-M2cd GYO reishi kit G. lucidum G. sessilech MH160057/MH168054
AL-M3sy GYO reishi kit G. lucidum G. lingzhi MH160058/MH168055
AL-M4cd GYO reishi kit G. lucidum G. sessilech MH160059/MH168056
AL-M5cd GYO reishi kit G. lucidum G. sessilech MH160060/MH168057
AL-M6sd GYO reishi kit G. lucidum G. lingzhi MH160061/MH168058
AL-M7cd GYO reishi kit G. lucidum s.l. G. resinaceum s.l.ch MH160062/MH168059
AL-M8cd GYO reishi kit G. lucidum G. curtisii MH160063/MH168060
AL-M9sd GYO reishi kit G. lucidum G. lingzhi MH160064/MH168061
AL-M10sd GYO reishi kit G. lucidum G. lingzhi MH160065/MH168062
AL-M11sy GYO reishi kit G. lucidum G. lingzhi MH160066/MH168063
AL-M12cd GYO reishi kit G. lucidum G. lingzhi MH160067/MH168064
AL-M13sd GYO reishi kit G. lucidum s.l. G. resinaceum s.l.ch MH160068/MH168065
AL-M14sd GYO reishi kit G. lucidum s.l. G. resinaceum s.l.ch MH160069/MH168066
AL-M15sd GYO reishi kit G. lucidum G. lingzhi MH160070/MH168067
AL-M16cd GYO reishi kit G. lucidum G. lucidum MH160071/MH168068
AL-M17sd GYO reishi kit G. curtisii G. curtisii MH160072/MH168069
AL-R1ca Manufactured product G. lucidum G. lingzhicu MH160073/-
AL-R2ca Manufactured product G. lucidum G. lingzhimfb MH160074/-
AL-R3ca Manufactured product G. lucidum G. lingzhicu MH160075/-
AL-R4ca Manufactured product G. lucidum/Lentinula edodes FAILEDifb -/-
AL-R5ca Manufactured product G. lucidum G. lingzhimfb MH160076/-
AL-R6ca Manufactured product G. lucidum G. applanatummfb MH160077/-
AL-R7ca Manufactured product G. lucidum G. lingzhisp MH160078/-
AL-R8ca Manufactured product G. lucidum G. lingzhiifb MH160079/-
AL-R9po Manufactured product G. lucidum G. lingzhisp MH160080/-
AL-R10po Manufactured product G. lucidum G. lingzhiifb MH160081/-
AL-R11ca Manufactured product G. tsugai5, G. lucidum, G. applanatum FAILEDcu -/-
AL-R12po Manufactured product G. lucidum FAILEDmfb -/-
AL-R13ca Manufactured product G. lucidum s.l. G. lingzhicu MH160082/-
AL-R14po Manufactured product G. lucidum G. lingzhisp MH160083/-
AL-R15co Manufactured product Ganoderma sp. G. lingzhind MH160084/-
AL-R16ta Manufactured product unknown FAILEDmfb -/-
AL-R17ca Manufactured product G. lucidum s.l. G. lingzhiifb MH160085/-
AL-R18ca Manufactured product G. lucidum FAILEDifb -/-
AL-R19ca Manufactured product G. lucidum G. lingzhimfb MH160086/-
AL-R20te Manufactured product G. lucidum FAILEDmfb -/-

Products in bold were correctly labeled. 1Sample name with subscripts that code for type of product. For GYO kits (AL-M#), “cd” is for “colonized dowel,” “sd” is for “sawdust spawn,” and “sy” is for “syringe spawn.” For manufactured products (AL-R#), “ca” is for “capsule,” “po” is for “powder,” “ta” is for “tablet,” “co” is for “coffee,” and “te” is for “tea.” 2Products were either grow-your-own (GYO) reishi kits sold by mushroom cultivation companies in the United States or manufactured products, which were easily purchased online as capsules, powders, tablet, coffee, or tea. 3Product labels were the Ganoderma or relevant taxa that were written on the ingredients or nutritional label(s). If not present on label then product labels are coded as “unknown.” 4Taxon identified was based on Sanger sequencing with ITS and tefla for GYO reishi kits and ITS only for manufactured reishi products. If labeled with FAILED, then the reaction did not work. Subscripts indicate information about each product. For the GYO kits (AL-M#) “ch” is for “chlamydospores present.” For manufactured products “cu” is for “culture,” “ifb” is for “immature basidiomata,” “mfb” if for “mature basidiomata,” “sp” is for “only spores,” and “nd” is for “nothing detected.” 5Presumably, this product meant to have G. tsugae not G. tsugai.