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. 2018 Jul 16;9:1557. doi: 10.3389/fmicb.2018.01557

Table 3.

Illumina sequence reads for the manufactured products showing only the relevant taxa, which were either found on the label of the product or were Ganoderma species detected with the next generation sequencing technology using ITS1.

Sample # Product Label1 Relevant Fungal Taxa Present Detected in Products2
G. applanatum s.l. G. australe G. curtisii G. lingzhi G. lucidum s.s. G. gibbosum G. resinaceum s.l. G. sessile G. sinense G. tuberculosum Lentinula edodes Total Reads3
AL-R1 G. lucidum 0 0 0 27714 0 18 0 0 0 0 0 27732
AL-R2 G. lucidum 0 0 0 40336 0 356 0 0 0 0 0 40692
AL-R3 G. lucidum 0 0 0 2931 0 197 0 209 0 0 0 3337
AL-R4 G. lucidum/L. edodes 0 0 0 4290 0 0 0 1046 0 0 54 5390
AL-R5 G. lucidum 0 0 0 50463 0 161 0 0 0 0 0 50624
AL-R6 G. lucidum 21822 0 0 0 0 0 0 0 0 0 0 21822
AL-R7 G. lucidum 0 0 0 134057 0 0 0 0 0 0 0 134057
AL-R8 G. lucidum 0 0 0 4326 0 18 0 0 0 0 0 4344
AL-R9 G. lucidum 0 0 0 894 0 3 0 35 0 3 0 935
AL-R10 G. lucidum 0 0 0 11818 0 84 0 370 77 0 0 12349
AL-R11 G. tsugai, G. lucidum, G. applanatum 9243 0 0 34613 0 0 0 8729 0 0 0 52585
AL-R13 G. lucidum s.l. 0 0 0 32176 0 23 14193 46 0 0 0 46438
AL-R14 G. lucidum 0 0 0 85395 0 0 0 0 0 0 0 85395
AL-R15 Ganoderma sp. 0 0 0 40955 0 24 0 0 0 0 0 40979
AL-R16 Ganoderma sp. 0 0 0 67340 0 42 0 144 0 0 0 67526
AL-R17 G. lucidum s.l. 19 19 0 55720 0 239 0 353 0 76 0 56407
AL-R18 G. lucidum 0 0 0 1797 0 286 0 0 0 0 0 2083
AL-R19 G. lucidum 0 0 0 4407 0 8 0 17 0 0 0 4415
AL-R20 G. lucidum 0 0 0 27215 0 563 0 682 0 0 0 28460
Pos. Ctrl4 G. curtisii, G. lingzhi, G. lucidum s.s., G. resinaceum s.l., G. sessile, G. tuberculosum 0 12822 12099 51020 0 36548 27460 0 6015 0 145964
Total Reads5 31084 12822 638546 51020 2022 50741 39074 77 6094 54 831534
Total Reads in Product6 31084 0 626447 0 2022 14193 11614 77 79 54 685570

1Product labels were the Ganoderma or relevant taxa that were written on the ingredients or nutritional label(s). 2Relevant taxa were those detected via Illumina sequencing method. 3Total Illumina sequence reads per the relevant taxa in each product. 4The positive control was a mock community spiked with equal volumes of each taxon at 5 ng/μl purified DNA. 5Total Illumina sequence reads for all products and positive control. 6Total Illumina sequence reads for all products minus the positive control.