Figure 2.
Effect of GHK on the amounts of spin signal adducts of ·OH, O2 -·, and t-BOO· generated by their respective chemical reaction systems. A. Representative ESR signal spectra of (a) DMPO-OH reflecting ·OH, (b) DMPO-OOH reflecting O2 -·, and (c) POBN adduct signal reflecting t-BOO·. (a) Fe2+, 0.25 mM; H2O2, 0.5 mM. (b) xanthine, 200 mM; XO, 0.1 U/mL. (c) Ce4+, 0.2 mM; t-BOOH, 400 mM. B. The effect of GHK on the amounts of spin signal adducts of ·OH, O2 -·, and t-BOO·. The radical intensity was defined as the ratio of the peak height of respective signal [indicated as arrows in part Aa-Ac] to that of Manganese (Mn). Results are presented as means ± standard errors of the mean (n = 3-8). α P < 0.01 vs. Control. GHK, 250 μM. ·OH, hydroxyl radicals; O2 -·, superoxide radicals; t-BOO·, tert-butyl peroxyl radicals; GHK, glycyl-ʟ-histidyl-ʟ-lysine; XO, xanthine oxidase; SOD, superoxide dismutase; ESR, electron spin resonance; t-BOOH, tert-butyl hydroperoxide.