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. 2018 Jun 29;115(29):E6880–E6889. doi: 10.1073/pnas.1719548115

Fig. 5.

Fig. 5.

Optic light stimulation activates sympathetic nerves and promotes anagen entry by enhancing norepinephrine release in skin. (A) Effect of light stimulation on renal sympathetic nerve activity. Sympathetic activity was increased by optic blue light stimulation in wild-type mice but not in Opn4−/− mice. Similar results were obtained in three independent experiments. (B) Effect of light stimulation on heart rates. The heart rate was increased by optic blue light stimulation in wild-type mice but not in Opn4−/− mice. Similar results were obtained in three independent experiments. (C) Effect of light stimulation on foot-pad sweating. Optic blue light stimulation induced sweating (blue arrowhead) in wild-type mice but not in Opn4−/− mice. Similar results were obtained in three independent experiments. (D) Effect of light on cutaneous norepinephrine release. Norepinephrine was highly increased after light irradiation in wild-type mice but not in Opn4−/− mice. *P < 0.05 compared with time 0 (n = 3). (E) Immunostaining. Enriched expression of β-2 adrenergic receptor (B2AR) in the HFSC compartment. A similar pattern of expression was observed in all HFs examined (n = 30). BG, bulge; SG, sebaceous gland; SHG, secondary hair germ. Dashed line represents dermal papilla. (Scale bar, 50 μm.) (F) Effect of β-adrenergic antagonist. Topical propranolol inhibited light-induced anagen entry. *P < 0.05 (n = 10).