Table 1.

Thermodynamic parameters obtained from Ising analysis

Construct	ΔGN	ΔGR	ΔGC	ΔGi−1,i	mGdn,i	mglycerol,i	mGdn,C	ΔGN,R
DHR10.2	1.46 [1.26, 1.67]	−2.51 [−2.90, −2.15]	0.63 [0.32, 1.00]	−4.80 [−5.10, −4.53]	−1.23 [−1.33, −1.14]	0.36 [0.33, 0.40]	N/A	N/A
DHR54	−0.45 [−0.58, −0.32]	−2.04 [−2.17, −1.92]	−0.84 [−0.94, −0.74]	−6.76 [−6.98, −6.54]	−1.24 [−1.28, −1.21]	0.41 [0.39, 0.43]	N/A	−7.72 [−7.95, −7.49]
DHR71	−3.01 [−3.27, −2.75]	−1.41 [−1.61, −1.23]	3.06 [2.87, 3.29]	−9.93 [−10.50, −9.43]	−1.57 [−1.66, −1.49]	0.17 [0.15, 0.20]	−0.71 [−0.79, −0.64]	N/A
DHR79	−1.84 [−2.06, −1.64]	−3.48 [−3.83, −3.22]	−1.81 [−2.08, −1.61]	−4.83 [−5.14, −4.55]	−1.12 [−1.18, −1.06]	0.15 [0.12, 0.18]	N/A	N/A

Free energies have units of kilocalories/mole. m_Gdn,i and m_glycerol,i have units of kilocalories/mole/[molar GdnHCl] and kilocalories/mole/[molar glycerol]. The 95% confidence intervals shown in brackets are from bootstrap analysis with 2,000 iterations. N/A, not applicable.