Fig. 5.

Inhibition of amyloid seeding by designed peptide inhibitors. (A) ThT assay to evaluate inhibition of seeding of ex vivo ATTR-D38A at pH 4.3 by 180 μM designed inhibitors of TTR aggregation when added to 0.5 mg/mL recombinant wild-type TTR. All replicates are shown; n = 3. (Right Inset) Anti-TTR dot blot of insoluble fractions. (B) Inhibition of amyloid seeding of 0.5 mg/mL MTTR by 180 μM TabFH2 at physiological pH, monitored by ThT fluorescence. All replicates are shown; n = 3. (C) Electron micrographs of samples containing TabFH2 obtained in A and B confirming inhibition of amyloid seeding.