Table 3.
Primer sequences for RT-qPCR
| Gene | Forward primer sequence (5′→3′) | Reverse primer sequence (5′→3′) | Access number | Product length, bp |
|---|---|---|---|---|
| ZFP423 | GGATTCCTCCGTGACAGCA | TCGTCCTCATTCCTCTCCTCT | NM_001101893.1 | 120 |
| PPARG | AGCTCCAAGAATACCAAAGTGCGAT | AGGTTCTTCATGAGGCCTGTTGTAGA | NM_181024.2 | 98 |
| ADH4 | AGAAAATGGGCACCAAGGGAA | TTCATTGCTGAGGGGCTTGT | NM_001102059.2 | 80 |
| ADH5 | TTGAGATGACTGACGGGGGA | CTCCCACTACCACGCTGATG | NM_001034249.2 | 117 |
| ALDH1A1 | GGAAATGGTCGAGAACTGGGA | GGAGGGCTTGGGTGTCATAG | NM_174239.2 | 299 |
| GAPDH | TGCCCGTTCGACAGATAGCC | GCGACGATGTCCACTTTGCC | NM_001034034.2 | 148 |
| β−atenin | CGGCTTTCGGTTGAGCTGAC | GCCGTACCCACCAGAGTGAA | XM_005222525.3 | 159 |
| 18S rRNA | CCTGCGGCTTAATTTGACTC | AACTAAGAACGGCCATGCAC | NR_036642.1 | 118 |