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. 2018 Jul 16;9:1601. doi: 10.3389/fmicb.2018.01601

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Copyright © 2018 Abdel-Motaal, Meng, Zhang, Abdelazez, Shao, Xu, Meng, Abozaed, Zhang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth tests for Escherichia coli KNabc and its transformants under saline or alkaline stress. For the complementation test (A), E. coli KNabc and its transformants on LBK medium plates with no addition of NaCl or LiCl, 0.2 M NaCl or 5 mM LiCl. 1, KNabc only; 2, KNabc/pUC18; 3, KNabc/pET19b; 4, KNabc/pET-truncated uvrD; 5, KNabc/pUC-PM29; 6, KNabc/pET-mdrP. Detailed growth tests for salt tolerance or alkaline pH resistance were performed by growing E. coli KNabc transformants carrying pET19b (white column) and pET-MdrP (black column) in LBK media with the addition of NaCl (B) or LiCl (C) at indicated concentrations or supplemented by 50 mM NaCl with the adjustment of pH (D) by Tris-HCl buffer at the final concentration of 100 mM. Cell growth was ended after 24 h and monitored turbidimetrically at 600 nm. Each data point represents the average of three independent determinations.