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. 2018 Jul 16;9:1601. doi: 10.3389/fmicb.2018.01601

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Copyright © 2018 Abdel-Motaal, Meng, Zhang, Abdelazez, Shao, Xu, Meng, Abozaed, Zhang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neighbor-joining phylogenetic tree of MdrP and its selected homologs, together with single-gene proteins with Na⁺/H⁺ antiport activity. For the construction of this phylogenetic tree, ten closest homologs with 60–95% identities, ten closer homologs with 40–59% identities and ten distant homologs with 29–39% identities, together with all characterized single-gene proteins with Na⁺/H⁺antiport activity were selected. Accession version numbers of selected homologs were shown in the parenthesis. Filled diamond stands for MdrP, open diamond stands for putative MFS homologs, filled triangle stands for known single-gene Na⁺/H⁺ antiporters, filled circle stands for other single-gene proteins with Na⁺/H⁺antiport activity. Bootstrap values ≥ 70% (based on 1000 replications) were shown at branch points. Bar, 0.1 substitutions per amino acid residue position.