Figure 2.

Dose‐dependent anti‐PS binding to PS in an immunoblot assay (A) Increasing concentrations of PS were subjected to SDS‐PAGE under non‐reducing conditions. Immunoblotting was performed with purified IgG from anti‐PS‐positive patient, or normal control (20 μg mL⁻¹). (B) PS (15 μg mL⁻¹) was subjected to SDS‐PAGE under non‐reducing conditions. Immunoblotting was performed with several dilutions of purified IgG from anti‐PS‐positive patient, or normal control. PS, protein S; SDS‐PAGE, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis