Figure 5.

Effects of Ca²⁺ in immunoblot for anti‐PS. (A) PS was subjected to SDS‐PAGE under reducing conditions. Immunoblots with normal plasma (control) or anti‐PS‐positive patient plasmas in the absence or presence of 2.0 mmol L⁻¹ of Ca²⁺. (B) PS was subjected to SDS‐PAGE under reducing conditions. Immunoblots with anti‐PS‐positive patient plasma in the presence of increasing concentrations of Ca²⁺. PS, protein S; SDS‐PAGE, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis