Table 2.

Comparison of substrate kinetics for the two main thrombin generation substrates

Substrate	H‐β‐Ala‐Gly‐Arg‐pNA9	Z‐Gly‐Gly‐Arg‐AMC8
Km	1.95 mmol L−1	0.31 mmol L−1
kcat	1.91 s−1	1.86 s−1
kcat/Km	9.8 × 102 M−1 s−1	6 × 103 M−1 s−1