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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Differentiation. 2018 Mar 27;101:16–24. doi: 10.1016/j.diff.2018.03.002

Fig. 4.

Fig. 4

Characterization of Hepatocyte-like cell differentiation. (A) Representative phase contrast micrograph of HLC cells (20 ×). (B) Expression of HNF4A, A1AT and ALBUMIN at 7 and 14 days, imaged with confocal microscopy. (C) Relative gene expression after 7 and 14 days of differentiation as measured by qRT-PCR. Data represent relative expression of transcripts normalized relative to GAPDH and undifferentiated controls. Data are represented as Mean ± SEM for three biologically independent experiments (n = 3). (D) Western blot assessment of HNF4A, A1AT and ALBUMIN expression at 7 and 14 days of HLC maturation. Representative data from three independent experiments are shown.