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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: Cell Host Microbe. 2018 Jun 13;23(6):775–785.e5. doi: 10.1016/j.chom.2018.05.004

Figure 5.

Figure 5

Tryptamine increases fluid secretion in colonoids. Representative example of Calcein-AM –labeled colonoids before and after application of tryptamine (1mM) for thirty minutes in GF (A) and HM (B) colonoids. Arrows represent colonoids that show prominent change in area. Change in surface area is measured relative to vehicle at t = 0 (Baseline; 100%). Change in surface area normalized to baseline (t=0) after application of vehicle (medium alone), media with tryptamine (1mM), media with tryptamine (1mM) co-applied with GR-113808 (100 nM), and media with forskolin (10 μM) in colonoids from GF male (C) and HM male (D) mice for thirty minutes from three independent wells. n=5, one-way ANOVA, *P < 0.05