Figure 3.

Expression of PAPSS isozymes during decidualisation. HESF were treated with either VC or DEC for 1–8 days as indicated and mRNAs encoding PAPSS1 and PAPSS2 were assessed by RT-qPCR. (A) PAPPS1 mRNA was significantly increased at 1 and 8 days in hESF treated with DEC compared to VC while PAPSS2 mRNA (B) was increased only after 1 day. n = 6 patients, duplicate treatments. Mann-Whitney test. *P < 0.05; **P < 0.01. DEC, decidualisation media; HESF, human endometrial stromal fibroblasts; PAPSS, adenosine 3′-phosphate 5′-phosphosulfate synthase; VC, vehicle control.