Analysis of lipid content in wild-type and osgpat3 mutant anthers. Amount per unit surface area (mg mm–2) of (A) cutin monomers, (B) internal lipid constituents, (C) wax constituents, and (D) total anther lipid contents in wild-type and osgpat3 anthers. Error bars indicate SD (n=4). Compound names are abbreviated as follows: C16 FA, palmitic acid; C18 FA, stearic acid; C18:1 FA, oleic acid; C18:2 FA, linoleic acid; C18:3 FA, linolenic acid; C20 FA, eicosanoic acid; C26 FA, cerotic acid; ALK, alkane; C16 ωHFA, 16-hydroxy-hexadecanoic acid; C18:1 ωHFA, 18-hydroxy-oleic acid; C18:2 ωHFA, 18-hydroxy-linoleic acid; cis-9,10-epoxy C18 ωHFA, cis-9,10-epoxy 18-hydroxy-stearic acid; cis-9,10-epoxy C18:1 ωHFA, cis-9,10-epoxy 18-hydroxy-oleic acid; methoxyhydrin of 9,10-epoxy C18 ωHFA, methoxyhydrin of 9,10-epoxy 18-hydroxy-stearic acid; chlorohydrin of 9,10-epoxy C18 ωHFA, chlorohydrin of 9,10-epoxy 18-hydroxy-stearic acid; C16-9/10, 16 DHFA, 9(10), 16-dihydroxy-hexadecanoic acid; C18-9/10, 18 DHFA, 9(10), 18-dihydroxy-stearic acid; C18:1–9/10, 18 DHFA, 9(10), 18-dihydroxy-oleic acid; C20 2HFA, 2-hydroxyeicosanoic acid; C20 DFA, eicosane-1, 20-dioic acid; C22 2HFA, 2-hydroxydocosanoic acid; C24 2HFA, 2-hydroxytetracosanoic acid. (This figure is available in colour at JXB online.)