Figure 6.
The importance of protein S for the anticoagulant effects of TFPIα and FV‐Short added to normal plasma. Thrombin generation was performed after addition of increasing concentrations of TFPIα to normal plasma without (A and B) or with (C and D) added FV‐Short and anti‐protein S (anti‐ProtS) (B and D). Thrombin generation was initiated with 1.4 pmol L−1 TF. The final concentrations of added TFPIα (nmol L−1) in the assay are indicated by the different colors in the figure. Note that the anticoagulant effects of TFPIα were dependent on protein S in particular in the presence of added FV‐Short. The experiment was performed multiple times with slight variations and a representative example is shown