Figure 4.

Dosing of mice with 50 mg kg⁻¹ 5SGlcNHex (12) reduced the O‐GlcNAc levels and impaired the secretion of the hormone leptin. a) Skeletal muscle from mice dosed with 50 mg kg⁻¹ of 12 showed a transient reduction in O‐GlcNAc levels by immunoblot analysis (CTD110.6). b) Leptin levels as measured by ELISA decreased in mice dosed with 12 to a minimum at 16 h. c) Dosing with 12 decreased O‐GlcNAc and Sp1 levels to a minimum at 16 h in fat pad tissue. d) Compound 12 lowered GlcNAc‐induced leptin secretion from 3T3‐L1 adipocytes. Results are given as the mean±SEM of three independent samples (n=3). Each independent sample was tested in duplicate; symbols denote statistical significance (*p<0.05, **p<0.01, ***p<0.001 compared to control; Student's t‐test).