Fig. 1.

Induction of PhOBF1 expression by TRV infection, abiotic stresses and hormone treatments in petunia leaves. (A, B) Quantitative real-time PCR analysis of PhOBF1 transcript levels in inoculated (A) and systemically infected upper (B) leaves with TRV at different time points. Four-week-old WT plants were inoculated with Agrobacterium bearing no TRV vector (mock control) or a TRV empty vector. (C, D) Quantitative real-time PCR analysis of PhOBF1 transcript levels in response to abiotic stresses (C) and hormones (D) at different time points. Three-week-old petunia seedlings were placed in vials with water at room temperature (control) or in a 4 °C room (cold), or without water at room temperature (dehydration), or with 100 mM NaCl, 50 μM ABA, 50 μM GA₃, 200 μM SA, or 200 μM MeJA, or exposed to 10 μl l⁻¹ ethylene treatment. Transcript abundances were standardized to 26S rRNA. Error bars represent standard error of the mean from three biological replicates.