Fig. 7.

Involvement of PhOBF1 in the biosynthesis of salicylic acid. (A, B) Free SA, SA glucoside (SAG), and phenylalanine levels in the leaves of WT, PhOBF1-RNAi (2, 6 and 8), and PhOBF1-overexpressing (OE) (B, D and H) lines. Samples were harvested from all the leaves of 4-week-old WT and transgenic seedlings. (C) Quantitative real-time PCR analysis of transcript abundances of genes, including DAHPS, SK1, EPSPS, CS, CM1, ADT1, PAL1, and PAL2, in the shikimate and phenylpropanoid pathways in the leaves of WT, PhOBF1-RNAi (2, 6 and 8), and PhOBF1-OE (B, D and H) lines. Samples were harvested from the uppermost leaves of 4-week-old WT and transgenic seedlings. Expression levels were normalized to 26S rRNA. Error bars represent standard error of the mean from three biological replicates. Asterisks indicate statistical significance using Duncan’s multiple range test at P<0.05.