Table 2. Top 20 shortlisted peptide sequence candidates with scores generated from human MHC class I and II epitope prediction parameters.

Sequence ID	Full sequence	KRAS mutation	Modified residue	MHC class IIa	MHC class Ib	Total scorec
68-D	YKLDVVGADGVGKSA	G12D	V7D	58	42	100
68-A	YKLDVVGAAGVGKSA	G12A	V7D	58	42	100
68-S	YKLDVVGASGVGKSA	G12S	V7D	58	42	100
68-V*	YKLDVVGAVGVGKSA	G12V	V7D	58	42	100
93-V	YKLVLVGAVGVGKSA	G12V	V8L	58	42	100
139-A	YKLVVVPAAGVGKSA	G12A	G10P	58	42	100
64-D	YKLYVVGADGVGKSA	G12D	V7Y	58	42	100
103-R	YKLVVAGARGVGKSA	G12R	V9A	58	42	100
116-R	YKLVVHGARGVGKSA	G12R	V9H	58	42	100
135-D	YKLVVVIADGVGKSA	G12D	G10I	58	42	100
138-S	YKLVVVKASGVGKSA	G12S	G10K	58	42	100
112-A	YKLVVNGAAGVGKSA	G12A	V9N	58	42	100
164-D*	YKLVVVGAGDVYKSA	G13D	G15Y	58	42	100
133-S	YKLVVVLASGVGKSA	G12S	G10L	58	42	100
135-A	YKLVVVIAAGVGKSA	G12A	G10I	58	42	100
84-S	YKLVYVGASGVGKSA	G12S	V8Y	58	42	100
78-D	YKLDVVGAVDVGKSA	G13D	V7D	58	42	100
224-D*	YKLVVVGAGDVGKSY	G13D	A18Y	58	42	100
67-D	YKLTVVGAGDVGKSA	G13D	V7T	58	42	100
194-D	YKLVVVGAGDVGQSA	G13D	K16Q	58	42	100
Control 1*	YKLVVVGAVGVGKSA	G12V	–	58	42	100
Control 2*	YKLVVVGAGDVGKSA	G13D	–	58	42	100
wtKRAS*	YKLVVVGAGGVGKSA	–	–	58	42	100

Notes:

Sequence ID denoted (*) were selected for immunogenicity assessment.

^aTotal score converted (as referred to Table 1) from the percentile score of human MHC class II epitope prediction generated from IEDB.

^bTotal score converted (as referred to Table 1) from the IC₅₀ score of human MHC class I epitope prediction generated from IEDB.

^cTotal score of ^a and ^b.