Table 3. Top 20 shortlisted peptide sequence candidates with scores generated from mouse MHC class I and II epitope prediction parameters.

Sequence ID	Full sequence	KRAS mutation	Modified residue	MHC class IIa	MHC class Ib	Total scorec
138-S	YKLVVVKASGVGKSA	G12S	G10K	58	28	86
84-S	YKLVYVGASGVGKSA	G12S	V8Y	39	42	81
135-A	YKLVVVIAAGVGKSA	G12A	G10I	39	28	67
139-A	YKLVVVPAAGVGKSA	G12A	G10P	39	28	67
93-V	YKLVLVGAVGVGKSA	G12V	V8L	39	28	67
78-D	YKLDVVGAVDVGKSA	G13D	V7D	39	28	67
164-D*	YKLVVVGAGDVYKSA	G13D	G15Y	39	28	67
68-A	YKLDVVGAAGVGKSA	G12A	V7D	20	42	62
68-V*	YKLDVVGAVGVGKSA	G12V	V7D	20	42	62
64-D	YKLYVVGADGVGKSA	G12D	V7Y	20	42	62
68-S	YKLDVVGASGVGKSA	G12S	V7D	20	42	62
103-R	YKLVVAGARGVGKSA	G12R	V9A	20	42	62
112-A	YKLVVNGAAGVGKSA	G12A	V9N	20	42	62
67-D	YKLTVVGAGDVGKSA	G13D	V7T	20	42	62
68-D	YKLDVVGADGVGKSA	G12D	V7D	20	28	48
133-S	YKLVVVLASGVGKSA	G12S	G10L	20	28	48
224-D*	YKLVVVGAGDVGKSY	G13D	A18Y	20	28	48
135-D	YKLVVVIADGVGKSA	G12D	G10I	20	28	48
116-R	YKLVVHGARGVGKSA	G12R	V9H	20	28	48
194-D	YKLVVVGAGDVGQSA	G13D	K16Q	20	28	48
Control 1*	YKLVVVGAVGVGKSA	G12V	–	20	28	48
Control 2*	YKLVVVGAGDVGKSA	G13D	–	20	28	48
wtKRAS*	YKLVVVGAGGVGKSA	–	–	20	28	48

Notes:

Sequence ID denoted (*) were selected for immunogenicity assessment.

^aTotal score converted (as referred to Table 1) from the percentile score of mouse MHC class II epitope prediction generated from IEDB.

^bTotal score converted (as referred to Table 1) from the IC₅₀ score of mouse MHC class I epitope prediction generated from IEDB.

^cTotal score of ^a and ^b.